Use of ammonium glycyrrhizinate_Kain Industrial Additives

Background and overview^[1][2]

Ammonium glycyrrhizinate has a strong sweetness, about 200 times as sweet as sucrose. It is usually used as a sweetener in food additives and is used in canned meats, seasonings, candies, biscuits, preserved fruits and beverages. . Monoammonium glycyrrhizinate has a strong affinity for sterol metabolism enzymes in the liver, thereby hindering the inactivation of cortisol and aldosterone. After use, it shows obvious corticosteroid-like effects, such as anti-inflammatory, anti-allergic and protective membrane structure. ; No obvious corticosteroid-like side effects.

This product can promote bile pigment metabolism, reduce the release of ALT and AST; induce γ-IFN and interleukin II, increase NK cell activity and OKT4/OKT8 ratio and activate the reticuloendothelial system; inhibit the release of histamine from mast cells ; Inhibit the formation and granulomatous reaction of cell membrane phospholipase A2 (PL-A2) and prostaglandin E2 (PGE2); inhibit the generation and formation of free radicals and peroxidized lipids, reduce the activity of prolyl hydroxylase; regulate calcium ions Channel, protect lysosomal membrane and mitochondria, reduce cell damage and necrosis; promote epithelial cells to produce mucopolysaccharides.

Preparation^[2]

It is made from natural licorice extracted with ammonia water and then refined with ammonium salt.

Toxicological basis^[2]

1. LD₅₀ mice take more than 10g/kg (bw) orally.

2. The bone marrow micronucleus test has no mutagenic effect.

Use ^[2-5]

Ammonium glycyrrhizinate can be used as a sweetener. Scope of use and usage:

(1) my country’s “Hygienic Standards for the Use of Food Additives” (GB2760-1996) stipulates that it can be used in canned meat, seasonings, candies, biscuits, preserved fruits and beverages in appropriate amounts according to production needs.

(2)FEMA. Regulations: The maximum usage amount is soft drinks, 51mg/kg; candy, 5.0~6.2mg/kg; baked goods, 5.0mg/kg.

(3) Actual usage reference: When used specifically, the dosage is chocolate, 0.4~0.98g/kg; biscuits, 0.25~0.6g/kg; orange juice, tomato juice, beverages, 0.4~0.7g/L, Add during topping. Wine, 0.3~0.5g/L, added after fermentation.

In addition, ammonium glycyrrhizinate can also be composed into a compound preparation. The combined compound preparation of ammonium glycyrrhizinate, glycine, and L-cysteine ​​hydrochloride has adrenocortical hormone-like effects but no hormone-like side effects; and by stabilizing cell membranes It exerts its anti-inflammatory and anti-allergic effects through various channels such as antagonizing allergic mediators; it can also be used for detoxification, viral hepatitis, and adjuvant treatment of tumor radiotherapy and chemotherapy. It can be used for allergic diseases: eczema, urticaria, rheumatism, allergic asthma, and allergies caused by sulfonamides, anesthetics and other drugs.

Poisoning by drugs and chemical substances: It has a detoxifying effect on poisoning by drugs or chemical substances such as benzene nitro compounds, benzene amino compounds, phenol, aromatic acids, heavy metals, etc. Intramuscular injection of this product before or at the same time as blood transfusion can prevent side effects such as fever, nausea, and vomiting. This product can treat toxic hepatitis, traumatic hepatitis, viral hepatitis and prevent liver necrosis. This product can prevent hematopoietic dysfunction, leukopenia, loss of appetite, fatigue, headache, nausea, vomiting, ascites, hair loss and other side effects caused by radiotherapy and chemotherapy, and can enhance its curative effect. Subcutaneous or intramuscular injection: 1 to 2 times a day, 1 to 2 tubes each time, half the dose for children or as directed by the doctor. Intravenous drip: Suitable for severe patients, once a day, 40-80m1 each time, dilute with 5% or 10% glucose injection and then infuse slowly, half the amount for children.

In addition, ammonium glycyrrhizinate can effectively protect the colon from damage in mice with inflammatory bowel disease. The mechanism may be related to enhancing the antioxidant capacity of the tissue. Compared with the current main drug for treating inflammatory bowel disease-Adisa, the efficacy is better. more significant. Diammonium glycyrrhizinate is mainly used clinically for the treatment of liver disease. Long-term clinical application has shown that it has a wide safety range, low toxic and side effects, no systemic side effects of hormones, and no immunosuppressive effects of immunosuppressants. The dosage of ammonium glycyrrhizinate used in the present invention to treat inflammatory bowel disease is within the range of commonly used clinical doses, indicating that ammonium glycyrrhizinate is not only effective in the treatment of inflammatory bowel disease, but also safe and has few side effects.

There is research and development of a traditional Chinese medicine compound injection and its preparation process, which includes total saponins of Panax notoginseng, ammonium glycyrrhizinate (monoammonium salt or diammonium salt), sodium chloride, glucose.�, mannitol, lactose, sorbitol, dextran and other clinically acceptable pH adjusters, osmotic pressure adjusters, freeze-drying excipients and water for injection. Injections, large-volume injections and freeze-dried powder injections are prepared from the above pharmacological ingredients. Each preparation unit contains 60 to 200 mg of Panax notoginseng total saponins and 80 to 200 mg of ammonium glycyrrhizinate.

Take the prescribed amount of Panax notoginseng total saponins and ammonium glycyrrhizinate, add appropriate amount of water, stir and heat in a water bath at 60°C to dissolve, adjust the pH value to 6.5~8.0, add activated carbon to decolorize and remove pyrogen, filter, and use water for injection After adding to the full amount, filter it through a microporous membrane, seal it in an ampoule, and sterilize it by autoclaving at 105°C. The invention can be used to treat chronic hepatitis, active liver cirrhosis, and prevent and treat liver fibrosis.

Content determination^[6]

A method for determining the content of ammonium glycyrrhizinate in Yinma Jiedu Granules, aiming to provide a method for determining the content of ammonium glycyrrhizinate with high detection accuracy and reliable detection results; the method is: 1) Weigh Yinma Jiedu Granules For 1g of particles, add 10g of diatomaceous earth and mix evenly for later use; 2) Extract; 3) Transfer the extract to an evaporating dish and evaporate it to dryness, dissolve it in acetonitrile and dilute it to a 10mL volumetric flask, add 2ml of the sample solution into a centrifuge tube for purification , the solution passes through a 0.22 micron filter membrane and then is injected into a liquid chromatograph for measurement; 4) Parameter settings for liquid chromatography analysis; it belongs to the field of chemical detection technology.

Main reference materials

[1] CN201410063364.4 Special ultra-fine powder freeze-dried preparation of compound ammonium glycyrrhizate and its preparation method

[2] Food Additive Manual

[3] Compound ammonium glycyrrhizinate injection

[4] Application of CN200610018369.0 ammonium glycyrrhizinate in the preparation of drugs for the treatment of inflammatory bowel disease

[5] CN200410087553.1 A traditional Chinese medicine compound injection and its preparation process

[6] CN201610259482.1 A method for determining the ammonium glycyrrhizinate content in Yinma Jiedu Granules